Free Download Ramanjulu Sunkar, "Plant Stress Tolerance: Methods and Protocols "
English | ISBN: 1071639722 | 2024 | 304 pages |
EPUB | 32 MB
This fully updated new edition explores new techniques for studying plant stress. This includes
novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase-seq for identifying nucleosome occupancy,
alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols,
growth-promoting bacteria isolation and
functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular
Biology series, chapters feature introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
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