Free Download Pamela K. Kreeger, "Ovarian Cancer: Methods and Protocols "
English | ISBN: 1071619551 | 2022 | 332 pages | EPUB | 43 MB
This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists, the book continues with sections exploring methods to characterize features of the tumor microenvironment (TME), techniques to isolate cells for in vitro study, and in vitro and in vivo models to study the disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Free Download Arash Kianianmomeni, "Optogenetics: Methods and Protocols "
English | ISBN: 1493935100 | 2016 | 461 pages | EPUB | 38 MB
This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic applications in neuroscience, brain mapping, treatment of neurological disorders, and restoration of visual function. Several introductory and discussion chapters offer a wide overview about sources and diversity of optogenetic tools, design strategies, and potential applications in other fields like plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Free Download Jeffrey M. Gross, "Ocular Regeneration: Methods and Protocols "
English | ISBN: 1071640860 | 2025 | 311 pages | EPUB | 53 MB
This detailed volume presents state-of-the-art techniques in ocular regeneration research, pulling together recent animal model systems identifying key genes and regulatory networks that contribute to the regenerative response as well as advances in induced pluripotent stem cell (iPSC) biology. The book opens with a section on the anterior segment and key models and techniques utilized to study the cornea, lens, and trabecular meshwork, and continues with chapters on the posterior segment, and largely the retina and retinal pigment epithelium (RPE), with techniques developed in a variety of model systems to study regeneration of these tissues. The concluding part examines novel technologies utilized in ocular regeneration research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.