Tag Archives: Methods

Plant Cytogenetics and Cytogenomics Methods and Protocols

July 2, 2025 by kenn in Ebooks - Leave a comment -
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Free Download Tony Heitkam, "Plant Cytogenetics and Cytogenomics: Methods and Protocols "
English | ISBN: 1071632256 | 2023 | 536 pages | EPUB | 58 MB
This book provides an up-to-date account of the most widespread methods used by specialists in the field of plant cytogenetics and the emerging field of cytogenomics that will likely soon be adapted by more labs. From the classical basic karyological approaches to the most recent genomics-informed and computational methods, the volume explores genome size and ploidy level estimation, chromosome fixation, preparation, and manipulation, banding and staining techniques, in situ hybridization, as well as numerous methods that integrate cytogenetics with bioinformatics and computational genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
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Phytophthora Methods and Protocols

July 2, 2025 by kenn in Ebooks - Leave a comment -
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Free Download Monica L. Gerth, "Phytophthora: Methods and Protocols "
English | ISBN: 1071643290 | 2025 | 261 pages | EPUB | 19 MB
This comprehensive volume examines techniques for studying Phytophthora species, a devastating group of plant pathogens that cause diseases in crops and natural ecosystems worldwide. The book compiles essential molecular techniques and methods, such as CRISPR-Cas genome editing for investigating pathogenicity and resistance, as well as tools for detecting, visualizing, and quantifying disease. Written for the highly successful Methods in Molecular Biology series, each chapter includes an introduction to its respective topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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NMDA Receptors Methods and Protocols

July 2, 2025 by kenn in Ebooks - Leave a comment -
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Free Download Nail Burnashev, "NMDA Receptors: Methods and Protocols "
English | ISBN: 1071638297 | 2024 | 305 pages | EPUB | 30 MB
This fully updated volume explores N-Methyl-D-aspartate receptors (NMDARs), and the structure-function relations, principles, and rules that govern how NMDARs operate in brain processing under normal and pathological conditions. With chapters from leading laboratories around the world, this book examines the expression and purification of diverse NMDA receptor subtypes, gene targeting and generation of conditional NMDAR mutant mouse lines, studies of NMDARs in zebrafish, electrophysiological studies, NMDARs modeling in silico, drug development with artificial intelligence, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Hepatocytes Methods and Protocols

July 2, 2025 by kenn in Ebooks - Leave a comment -
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Free Download Naoki Tanimizu, "Hepatocytes: Methods and Protocols "
English | ISBN: 107162556X | 2022 | 293 pages | EPUB | 28 MB
This volume details experimental protocols to study structure and function of hepatocytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepatoblastoma and identifying hepatocellular carcinoma subtypes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
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Electrophysiology Basics, Methods, Modern Approaches and Applications

July 2, 2025 by kenn in Ebooks - Leave a comment -
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Free Download Jürgen Rettinger, "Electrophysiology: Basics, Methods, Modern Approaches and Applications"
English | ISBN: 3030864812 | 2022 | 233 pages | EPUB | 27 MB
This updated and revised textbook presents a broad overview on topics concerning cellular electrophysiology – ranging from bioelectric phenomena recognized as far back as ancient Egypt to popular topics on the dangers of electrosmog. Without sacrificing scientific precision, this clear and concise work presents on the one hand the different methods and modern applications, on the other hand the biophysical fundamentals of ion-channel and carrier proteins. Numerous and carefully selected illustrations and diagrams supplement the text. Each chapter is extended by a detailed table of Take-Home Messages. Questions at the end of each chapter allow readers to test their understanding. Each section also includes references to relevant original literature for further reading. The book offers a valuable resource for students of biology, chemistry and physics with a special interest in biophysics.
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